Explain the role of reverse transcriptase in RT-PCR.

Explain the role of DNA polymerase in RT-PCR.

Any DNA in the sample is hydrolysed by enzymes before the sample is added to the reaction mixture.

Explain why.

A quantitative comparison can be made of the amount of RNA in samples A and B. This involves d

Suggest one reason why DNA replication stops in the polymerase chain reaction.

Scientists have used the RT-PCR method to detect the presence of different RNA viruses in patients suffering from respiratory diseases.

The scientists produced a variety of primers for this procedure.

Explain why.