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2019 · Paper 1 · Question 6 · 10 marks

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Figure for question 6
6.1

Describe and explain the data in Table 4.

2 marks
6.2

The scientists determined the percentage of heart cells undergoing DNA replication by using a chemical called BrdU. Cells use BrdU instead of nucleotides containing thymine during DNA replication.

Describe how BrdU would be incorporated into new DNA during semi-conservative replication.

5 marks
6.3

Cells with BrdU in their DNA are detected using an anti-BrdU antibody with an enzyme attached.

Use your knowledge of the ELISA test to suggest and explain how the scientists identified the cells that have BrdU in their DNA.

3 marks