2019 · Paper 1 · Question 6 · 10 marks
Save
Describe and explain the data in Table 4.
The scientists determined the percentage of heart cells undergoing DNA replication by using a chemical called BrdU. Cells use BrdU instead of nucleotides containing thymine during DNA replication.
Describe how BrdU would be incorporated into new DNA during semi-conservative replication.
Cells with BrdU in their DNA are detected using an anti-BrdU antibody with an enzyme attached.
Use your knowledge of the ELISA test to suggest and explain how the scientists identified the cells that have BrdU in their DNA.