Describe how enzymes could be used to insert the GH gene into a plasmid.

Microinjection of DNA into fertilised egg cells is a frequent method of producing transgenic fish. However, the insertion of the transferred gene into nuclear DNA may be delayed. Consequently, the offspring of transgenic fish may not possess the desired characteristic.

Suggest and explain how delayed insertion of the GH gene could produce offspring of transgenic fish without the desired characteristic.

Using Table 3, what can you conclude about the effectiveness of the GH gene on the growth of zebrafish?

Explain how two features of the design of this investigation helped to ensure the validity of any conclusions obtained.

Do not include calculating the mean or SD in your answer.

1

2